Part:BBa_K277074

3L.3_23.C2.04

3L.3_23.C2.04 is 788 bases long and is cloned into the pGem-T vector.

3L.3_23.C2.04 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. The whole synthetic chromosome may be viewed at http://macbeth.clark.jhu.edu/cgi-bin/gbrowse 3L.3_23.C2.04 is a constituent of 3L.3_23.C2 (along with 3L.3_23.C2.01, 3L.3_23.C2.02, 3L.3_23.C2.03, 3L.3_23.C2.05, 3L.3_23.C2.06, 3L.3_23.C2.07, 3L.3_23.C2.08, and 3L.3_23.C2.09.)

This part contains at least part of the following features (positions offset from first base of sequence):

kind and name offset notes

forward_primer YCL032W_tagf1v1 (388..415)

reverse_primer YCL032W_tagr1v1 (571..595)

gene YCL032W (-133..+907) Protein involved in mating response%2C invasive%2Ffilamentous growth%2C and osmotolerance%2C acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction

Sequence (corresponds to coordinates 54118..54905 in synthetic chromosome yeast_chr3_3_23)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 67
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 324